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KMID : 0525720120170010030
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Journal of Chitin and Chitosan
2012 Volume.17 No. 1 p.30 ~ p.36
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ntioxidant activity of carboxymethylated chitooligosaccharide
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Lee Jung-Kwon
Qian Zhoong?Ji
Byun Hee?Guk
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Abstract
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In this study, the antioxidative effects of carboxymethyl-chitooligosaccharide (CM-COS) on the generation of direct free radical scavenging activity was examined using an electron spin resonance (ESR) spectrometer and assess the cytotoxicity of CM-COS. Antioxidative activities of deacetylated COS at 70% (CM-COS-70) and 80% (CM-COS-80) were measured, respectively. In the ESR analysis, the CM-COS-80 was found to have the highest antioxidant activity when compared to the other CM-COS. The radical scavenging activities (effective concentration at 50%, EC50 (mg/mL)) of the CM-COS-80 were 3.29, 6.25, 2.65 and 1.50 mg/mL for DPPH, hydroxyl, alkyl and superoxide, respectively. Moreover, CM-COS did not show any cytotoxic effect against the MRC-5 cell line. In addition, the CM-COS-70 and -80 significantly inhibited oxidative damage to purified genomic DNA. Therefore, CM-COS derivatives may be used as a source of antioxidants, potentially as a food supplement, or by the pharmaceutical industry.
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KEYWORD
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Chitooligosaccharide, Carboxymethylation, Radical scavenging activity, Electron spin resonance
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